Sample Preparation / Nucleic Acid Extraction from Biological Samples

2011-2014

In order to provide easy access to diagnostic health care systems the project aims to create a low-cost, reliable, automated sample preparation system that can be interfaced with nucleic acid detection techniques like real-time PCR & LAMP, thus enabling creation of PoC molecular diagnostic devices. The technique involves the creation of a device to extract pathogenic DNA/RNA from biological samples such as blood, sputum, urine, & nasal/throat swabs.

Project Partners

Bigtec labs Pvt. Ltd. Bangalore, India

Indian Institute of Science, Bangalore India

University of Waterloo, Waterloo, Canada

Contact

Project Coordinator:

B. Chandrasekhar Nair, Bigtec labs Pvt. Ltd., Bangalore/India

Email bc at bigtec.co.in

Indian Academic Institution Partner:

Prof. D. Roy Mahapatra, Department of Aerospace Engineering, Indian Institute of Science, Bangalore/India

Email droymahapatra at aero.iisc.ernet.in

Canadian Academic Institution Partner:

Prof. John Yeow, Systems Design Engineering, University of Waterloo, Waterloo/Canada

Email jyeow at uwaterloo.ca

Research Funding

Grand Challenge Canada Foundation

Publication

Detection of target DNA using photo-reactive protoporphyrin moeity on a nanocomposite substrate, S Das, M Mishra, R Vasireddi, DR Mahapatra, SPIE Photonics West 2014, Proc. SPIE 8933, Frontiers in Biological Detection: From Nano-sensors to Systems VI, (March 13, 2014); doi:10.1117/12.2042556, 893308-6, February 1-6, 2014.

Mechanism of Cell Lysis in Microfluidic Channel with Integrated Nanocomposite Electrodes, Madhusmita Mishra, Anil Krishna Koduri, Aman Chandra, Roy D Mahapatra, GM Hegde, 2nd ASME Global Congress on Nanoengineering for Medicine and Biology, FEB 04-06, 2013, Boston, pp. 17-22.

Charge injection through nanocomposite electrode in microfluidic channel for electrical lysis of biological cells, M Mishra, A Krishna, A Chandra, BM Shenoy, GM Hegde, DR Mahapatra, SPIE BiOS, San Francisco, California, USA, February 02, 2013, Proc. SPIE 8570, Frontiers in Biological Detection: From Nanosensors to Systems V, 85700R (March 5, 2013); doi:10.1117/12.2012295.